3. I estimate that there were about 1000 bacteria in the 100 ul of bacteria that we spread on the plate. Since there were about 200 colonies in our +pGLO Lb/Amp/Ara plate, I estimated that there had to be much more bacteria that hadn't been transformed/not received the plasmid with resistance to ampicillin.
4. The role of arabinose in the plates is to make the bacteria glow under UV light. The arabinose activates the GFP gene which causes the bacteria to glow.
5. Three current uses for GFP in research or applied science are acting as a cell marker, tagging genes for movement of certain cancers, and showing promoter activity. A cell marker can be used to track where bacteria is present and tagging genes can be used to show the movement of certain cancers. GFP can also be used to show promoter activity in operons.
6. Another application of genetic engineering is how plants are altered to express a gene that isn't native to that particular plant or to modify its genes. It can be used to provide resistance to drought or extreme temperature.
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